Figure 5.

Immunohistochemistry analyses were performed to study infiltrating lymphocytes (TILs) in tumors. A. Tumor tissue sections were separately incubated with anti-CD4, anti-CD8 and anti-CD57 mAbs overnight at 4°C. Sections were then incubated with biotinylated anti-mouse antibody and streptavidin-biotinylated peroxidase complex. B. Intensity of lympocyte infiltration in the tumors was determined by the mean positive cell counts in the dermis around the B16-NY-ESO-1 tumor per field (10 randomly selected high power fields/slide). For each site, 3 pathologists performed a blind read of the glass slides.