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GAD1 gene expression in 1 dpf forebrain following transient ethanol exposure. A, control untreated embryo; B–D, embryos exposed to ethanol from 5.25–6.25 hpf; E–G, embryos exposed to 3% or 5% ethanol from 8–10 hpf. H, quantitation of decreased GAD1 expression in ethanol exposed embryos. Data are shown as Mean ± SD for at least 3 independent experiments, with total number of embryos analyzed shown. **, significantly different from control GAD1 expression, P< 0.0001.
