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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Neurotoxicol Teratol. 2014 Jun 11;44:70–80. doi: 10.1016/j.ntt.2014.06.001

Figure 8.

Figure 8

Correlation between ethanol-induced morphological changes and reduced neuronal marker gene expression. A–E, Pax6a gene expression in 2 dpf eye, showing wild-type gene expression (A), embryos exposed to ethanol with normal Pax6a expression (B,D) and embryos displaying decreased Pax6a expression after ethanol exposure (C,E). The white bars indicate the diameter of the eye and allow comparison between decreased eye diameter (C,E) and unchanged eye diameter (B,D) when compared to control (A). F–O, GAD1 gene expression in sorted 2 dpf embryos, showing normal expression in control (F,K), and 3% ethanol exposed (G,I,L,N). P, correlation of eye diameter to gene expression in 2 dpf embryos. Data are shown as Mean ± SD for at least 3 independent experiments, with total number of embryos analyzed shown. **, significantly different from control eye size, P< 0.0001.