Fig. 9.

Medial nucleus of the trapezoid body- (MNTB-) evoked synaptic Ca²⁺ responses in neonatal lateral superior olive (LSO) neurons. (A) Left column: responses in four LSO neurons to single electrical shocks applied to the ipsilateral MNTB. Middle column: responses to five shocks delivered at 20 Hz. Right column: responses to a single shock in the presence of 20 mM TEA. (B) Pharmacology of synaptic Ca²⁺ responses of two neurons in the medial limb of the LSO (P4 mouse). Both cells responded to stimulation of the MNTB and one cell responded to stimulation of the ipsilateral ventral acoustic stria (iVAS). Blocking ionotropic and metabotropic glutamate receptors with CNQX (10 μM), APV (100 μM), and MCPG (250 μM) abolished iVAS-evoked responses. Blocking glycine and GABA receptors with strychnine and bicuculline (10 μM each) reversibly abolished MNTB-evoked responses. Pseudocolour images were calculated from images just before stimulation (baseline) and at peak of responses.