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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Arch Oral Biol. 2014 Jun 14;59(10):1056–1064. doi: 10.1016/j.archoralbio.2014.06.003

Figure 1. Zymograms showing gelatinase activity of T. denticola dentilisin protease, pro-MMP-2 and activated MMP-2.

Figure 1

PDL cells were challenged with T. denticola at MOI=100 for 2h, washed twice in PBS and incubated in serum- and antibiotic-free medium with daily changes. Panel A: Gelatinase activity in equal volumes of conditioned medium collected on the indicated days following T. denticola challenge. Panel B: Gelatinase activity in lysates of PDL cells collected on the indicated days following T. denticola challenge and medium replacement as in Panel A. Equal amounts of protein were loaded per lane. The locations of the active dentilisin complex (95-100 kDa), 72-kDa pro-MMP-2 and 64-kDa activated MMP-2 are indicated, as are the positions of relative molecular mass markers in kDa.