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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Arch Oral Biol. 2014 Jun 14;59(10):1056–1064. doi: 10.1016/j.archoralbio.2014.06.003

Figure 4. Analysis of MMP-2 promoter methylation in PDL cells following T. denticola challenge.

Figure 4

PDL cells were challenged with T. denticola for 2h, washed, and incubated in fresh medium for 24 h prior to analysis. The vertical scale indicates percent of total DNA. Panel A: methylation analysis of the MMP-2 promoter region as determined by MethylDNA Restriction Screen (Qiagen). Results are expressed as the percentage of hyper-methylated and unmethylated DNA, which sum to 100%. Data were analyzed using Student's t-test. Panel B: DNA methylation analysis of the MMP-2 promoter region as determined by sequencing of independent PCR clones of bisulfite-converted DNA from T. denticola challenged and unchallenged PDL cell cultures. Each circle represents an individual CpG within residues 236-456 of MMP2-exon 1-transcript variant 1. Open and closed circles indicate unmethylated and methylated CpGs, respectively within residues 236-456 of MMP2-exon 1.