| User aliquots with high divalent cation concentrations (4 mM Ca2+, 4 mM Mg2+): | |
|---|---|
| Reagent | Quantity (for 0.5 L) |
| ACSF solution stock | 0.5 mL |
| calcium chloride, CaCl2, 1 M stock solution | 1.0 mL |
| magnesium chloride, MgCl2, 1 M stock solution | 0.5 mL |
| Storage: 4 °C. Shelf life: up to 1 week | |
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