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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Dev Biol. 2014 May 13;392(1):42–51. doi: 10.1016/j.ydbio.2014.05.001

Figure 3. Increased incidence of Activated Caspase 3-positive oocytes and somatic cells in PND 0 TAF4b (−/−) ovaries.

Figure 3

A) Activated Caspase 3 (red) and Tra98 (green) staining was performed in 8μm PND 0 ovary tissue sections. TAF4b (−/−) ovaries (right panel) possess far greater numbers of Activated Caspase 3- positive oocytes relative to TAF4b (+/+) ovaries (left panel). Inset: High magnification insets denote Activated Caspase 3-positive germ cells (red arrows), Activated Caspase 3-positive somatic cells (white arrow), and pyknotic nuclei (yellow arrowheads) in TAF4b (−/−) ovaries, which are absent from TAF4b (+/+) ovaries. Green arrows denote non-apoptotic oocytes. B) Quantification of apoptotic oocytes. Cells positive for both Activated Caspase 3 and Tra98 were counted per section and divided by the total number of Tra98-positive cells per section. ***: n=3, two-tailed T-test, p<0.001.