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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Dev Biol. 2014 May 13;392(1):42–51. doi: 10.1016/j.ydbio.2014.05.001

Figure 4. Ex vivo-cultured PND 1 ovaries recapitulate in vivo oocyte depletion.

Figure 4

A) E18.5 TAF4b (+/−) and (−/−) ovaries were harvested and cultured until PND 1 in ovary culture media. Ovaries were then embedded and stained for Tra98 (green) to assess germ cell numbers. Cultured TAF4b (−/−) experience oocyte depletion similar to in vivo TAF4b (−/−) ovaries (right panel), while cultured TAF4b (+/−) ovaries maintain the oocyte reserve (left panel). B) Representative sections of in vivo PND 2 TAF4b (+/+) and (−/−) ovaries.