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. 2014 Jul 12;4(7):e005318. doi: 10.1136/bmjopen-2014-005318

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Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

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Cross-sectional analysis of the methylation rates of the glucocorticoid receptor (GR) gene promoter in leucocytes determined using the Mquant method. Percentage of methylation at each CpG site (mean±SEM) in the GR promoter isolated from peripheral blood collected at birth and on postnatal day 4 in term (dark grey bar) and preterm (light grey bar) infants, respectively. Mann–Whitney U tests were performed to compare the methylation rates in preterm and term infants at each CpG site. A p value <0.05 (two tailed) was considered statistically significant. Closed star symbol, methylation rate significantly higher in preterm compared with term infants; Open star symbol, methylation rate significantly lower in preterm compared with term infants.