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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Neurobiol Dis. 2014 May 27;69:124–133. doi: 10.1016/j.nbd.2014.05.018

Figure 5.

Figure 5

IL-1β overexpression reduces amyloid pathology in the absence of CCR2 without altering APP or processing. A,B, Representative images of 6E10 (A) and Congo red (B) staining of amyloid plaques in 7 mo old APP/PS1/ CCR2-/- mice transduced with either rAAV2-Phe or rAAV2-IL-1β. Scale bar = 30 µm. C,D, Quantification of 6E10 (C) and Congo red (D) displayed as percent area of hippocampus covered by amyloid plaques. E,F,G,H, ELISA measurements of hippocampal levels of insoluble Aβ1-42 (E), soluble Aβ1-42 (F), insoluble Aβ1-40 (G), and soluble Aβ1-40 (H). n = 8-9 per group. Data displayed as mean ± SEM, unpaired t-test, ***p <0.0001, **p < 0.005, *p < 0.05. I, Representative Western blot images and quantification of band intensities for APP, BACE, and β-CTF normalized to tubulin. n = 6-8 per group. Graph bars represent mean ± SEM, Mann-Whitney test.