Table 1.
(A) mRNA expression of inflammatory mediators in the frontal cortex (area 8) of control and sCJD subtypes MM1 and VV2. (B) mRNA expression of inflammatory mediators in the cerebellum of control and sCJD subtypes MM1 and VV2.
| ID | Control |
sCJD MM1 |
sCJD VV2 |
|||||
|---|---|---|---|---|---|---|---|---|
| F Change | n | F Change | p | n | F Change | p | n | |
| A. FRONTAL CORTEX | ||||||||
| Anti-inflammatory cytokines | ||||||||
| TGFB family | ||||||||
| TGFB1 | 1.07 ± 0.11 | 14 | 2.92 ± 0.55 | ** | 15 | 1.82 ± 0.17 | 14 | |
| TGFB2 | 1.05 ± 0.10 | 14 | 2.25 ± 0.39 | ** | 14 | 1.83 ± 0.20 | 14 | |
| IL10 family | ||||||||
| IL10 | 1.28 ± 0.24 | 13 | 3.21 ± 0.53 | ** | 14 | 2.17 ± 0.27 | 15 | |
| IL10RA | 1.06 ± 0.10 | 14 | 2.95 ± 0.53 | *** | 14 | 2.01 ± 0.23 | 15 | |
| IL10RB | 1.07 ± 0.11 | 14 | 2.64 ± 0.35 | *** | 14 | 1.58 ± 0.15 | ## | 15 |
| Pro-inflammatory cytokines | ||||||||
| IL6 | 1 ± 0.21 | 11 | 2.21 ± 0.32 | * | 12 | 1.72 ± 0.34 | 13 | |
| IL6st | 1.04 ± 0.09 | 14 | 1.46 ± 0.13 | * | 14 | 1.17 ± 0.08 | 15 | |
| IL8 | 1.41 ± 0.32 | 14 | 1.78 ± 0.67 | 14 | 0.94 ± 0.17 | 14 | ||
| IL1B | 1.44 ± 0.32 | 13 | 2.41 ± 0.55 | 14 | 1.96 ± 0.39 | 15 | ||
| TNFA-family | ||||||||
| TNFA | 1.00 ± 0.27 | 9 | 5.27 ± 1.28 | ** | 14 | 2.36 ± 0.33 | # | 14 |
| TNFRSF1A | 1.13 ± 0.16 | 14 | 3.21 ± 0.45 | *** | 14 | 2.50 ± 0.24 | ** | 15 |
| Complement system | ||||||||
| C1QL1 | 1.11 ± 0.17 | 14 | 1.48 ± 0.19 | 15 | 1.33 ± 0.12 | 14 | ||
| C1QTNF7 | 1.13 ± 0.18 | 14 | 1.18 ± 0.17 | 15 | 0.82 ± 0.08 | 15 | ||
| C3AR1 | 1.10 ± 0.12 | 14 | 5.82 ± 0.83 | *** | 15 | 2.31 ± 0.36 | ### | 14 |
| Integrin family & CTL/CTLD superfamily | ||||||||
| CLEC7A | 1.10 ± 0.12 | 14 | 2.90 ± 0.56 | ** | 15 | 1.36 ± 0.19 | # | 14 |
| ITGB2 | 1.27 ± 0.25 | 14 | 5.75 ± 1.11 | *** | 15 | 3.34 ± 0.57 | 15 | |
| CST7 | 1.55 ± 0.43 | 14 | 2.32 ± 0.94 | 15 | 1.30 ± 0.30 | 15 | ||
| CYBA | 1.18 ± 0.18 | 14 | 3.20 ± 0.56 | ** | 14 | 1.95 ± 0.30 | 15 | |
| INPP5D | 1.09 ± 0.11 | 14 | 2.93 ± 0.71 | * | 15 | 1.58 ± 0.19 | 15 | |
| TLRs | ||||||||
| TLR4 | 1.05 ± 0.08 | 14 | 2.22 ± 0.30 | * | 14 | 1.77 ± 0.23 | 15 | |
| TLR7 | 1.20 ± 0.22 | 14 | 3.36 ± 0.49 | *** | 14 | 2.24 ± 0.37 | 15 | |
| Colony stimulating factors | ||||||||
| CSF1R | 1.10 ± 0.13 | 14 | 2.60 ± 0.47 | ** | 15 | 1.49 ± 0.14 | # | 15 |
| CSF3R | 1.17 ± 0.19 | 14 | 3.43 ± 0.61 | *** | 15 | 1.70 ± 0.19 | ## | 15 |
| Cathepsins | ||||||||
| CTSC | 1.17 ± 0.18 | 14 | 4.64 ± 0.89 | *** | 15 | 2.93 ± 0.41 | 14 | |
| CTSS | 1.11 ± 0.15 | 14 | 4.29 ± 0.83 | *** | 15 | 2.47 ± 0.46 | 15 | |
| B. CEREBELLUM | ||||||||
| Anti-inflammatory cytokines | ||||||||
| TGFB family | ||||||||
| TGFB1 | 1.09 ± 0.12 | 15 | 4.29 ± 0.79 | *** | 14 | 3.44 ± 0.43 | * | 11 |
| TGFB2 | 1.05 ± 0.09 | 15 | 2.77 ± 0.48 | ** | 15 | 3.01 ± 0.45 | ** | 11 |
| IL10 family | ||||||||
| IL10 | 1.15 ± 0.17 | 15 | 3.05 ± 0.77 | * | 13 | 7.67 ± 1.40 | *** ## | 10 |
| IL10RA | 1.24 ± 0.22 | 14 | 3.54 ± 0.59 | 13 | 11.67 ± 2.12 | *** ### | 11 | |
| IL10RB | 1.08 ± 0.12 | 14 | 2.92 ± 0.36 | ** | 14 | 3.85 ± 0.51 | *** | 11 |
| Pro-inflammatory cytokines | ||||||||
| IL6 | 1 ± 0.23 | 12 | 1.52 ± 0.27 | 14 | 2.35 ± 0.54 | * | 9 | |
| IL6st | 1.07 ± 0.11 | 14 | 1.84 ± 0.18 | * | 14 | 2.21 ± 0.33 | ** | 11 |
| IL8 | 1.33 ± 0.27 | 14 | 3.44 ± 0.85 | * | 14 | 1.70 ± 0.35 | 10 | |
| IL1B | 1.11 ± 0.15 | 13 | 2.08 ± 0.38 | 14 | 3.10 ± 0.59 | ** | 10 | |
| TNFA-family | ||||||||
| TNFA | 1.00 ± 0.15 | 14 | 2.20 ± 0.43 | * | 13 | 3.84 ± 0.66 | *** # | 10 |
| TNFRSF1A | 1.10 ± 0.13 | 15 | 2.92 ± 0.36 | * | 14 | 4.69 ± 0.81 | *** # | 11 |
| Complement system | ||||||||
| C1QL1 | 1.15 ± 0.16 | 15 | 2.07 ± 0.34 | 14 | 2.53 ± 0.39 | ** | 11 | |
| C1QTNF7 | 1.08 ± 0.11 | 15 | 1.36 ± 0.27 | 13 | 2.62 ± 0.56 | ** # | 11 | |
| C3AR1 | 1.19 ± 0.20 | 15 | 4.68 ± 0.91 | ** | 14 | 8.30 ± 1.26 | *** # | 10 |
| Integrin family & CTL/CTLD superfamily | ||||||||
| CLEC7A | 1.09 ± 0.11 | 14 | 2.13 ± 0.45 | 13 | 3.28 ± 0.48 | *** | 10 | |
| ITGB2 | 1.15 ± 0.19 | 14 | 5.25 ± 1.21 | * | 14 | 6.07 ± 1.29 | ** | 11 |
| CST7 | 1.30 ± 0.26 | 14 | 1.50 ± 0.43 | 15 | 1.97 ± 0.46 | 11 | ||
| CYBA | 1.24 ± 0.23 | 15 | 5.20 ± 1.18 | ** | 15 | 6.66 ± 0.96 | *** | 11 |
| INPP5D | 1.15 ± 0.16 | 15 | 3.14 ± 0.63 | * | 14 | 3.55 ± 0.66 | ** | 11 |
| TLRs | ||||||||
| TLR4 | 1.09 ± 0.11 | 15 | 3.73 ± 0.61 | *** | 15 | 2.33 ± 0.29 | 11 | |
| TLR7 | 1.17 ± 0.22 | 15 | 3.28 ± 0.57 | * | 15 | 5.65 ± 1.14 | *** # | 10 |
| Colony stimulating factors | ||||||||
| CSF1R | 1.07 ± 0.10 | 15 | 2.37 ± 0.50 | 14 | 3.83 ± 0.68 | *** | 10 | |
| CSF3R | 1.10 ± 0.12 | 14 | 2.73 ± 0.54 | 14 | 5.90 ± 1.34 | *** # | 10 | |
| Cathepsins | ||||||||
| CTSC | 1.10 ± 0.14 | 15 | 2.94 ± 0.58 | 13 | 5.22 ± 1.05 | *** # | 11 | |
| CTSS | 1.06 ± 0.09 | 15 | 3.96 ± 0.70 | ** | 14 | 6.49 ± 1.06 | *** # | 11 |
Data are expressed as the mean SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 sCJD compared with control cases. #p < 0.05, ##p < 0.01, ###p < 0.001 sCJD VV2 compared with sCJD MM1 cases (ANOVA one-way, Tukey’s post hoc test).