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. Author manuscript; available in PMC: 2014 Aug 4.
Published in final edited form as: Anal Biochem. 2011 Apr 6;414(2):282–286. doi: 10.1016/j.ab.2011.03.030

Figure 1.

Figure 1

(A) Self-assembled protein microarray scheme using a multiple spotting technique. First, Anti-GFP is printed on a conductive glass slide coated with an amine-reactive surface and the slide is incubated in a humid chamber to allow for immobilization of the antibody. DNA constructs with a T7 promoter and C-terminal GFP tag are printed at each microarray element on top of the immobilized antibody. Cell-free extract is printed on top of the DNA/antibody spots and rehydration of the spots commences transcription, translation, and capture of the full-length bait protein. (B) Rehydration of the microarray in a sealed chamber forms separate protein synthesis reactions at each array element.