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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Neurochem Int. 2014 Apr 18;72:14–21. doi: 10.1016/j.neuint.2014.04.006

Figure 1. BDNF-Dependent Increase in Gephyrin Protein Expression.

Figure 1

Starting at 7–8 DIV, primary cultured hippocampal neurons were incubated with BDNF (50 ng/ml) for 1 or 2 days to analyze gephyrin protein expression. (A) Representative western blots of gephyrin, NSF, or actin are shown. (B) Densitometry analysis of gephyrin or NSF immunoreactivity is presented as the mean ± S.E.M. of 5 independent experiments. Gephyrin or NSF immunoreactivity was normalized to the corresponding actin signal. BDNF application increased the protein expression of gephyrin (*p<0.05 compared to control by ANOVA) but not that of NSF. (C) To test if the change in gephyrin protein expression was mediated by BDNF, TrkB-Fc was added to sequester BDNF from the extracellular media. Neurons were incubated with BDNF (50 ng/ml) and/or TrkB-Fc (2 μg/ml) for 2 days and gephyrin protein expression was analyzed. Representative blots for gephyrin, NSF, or actin are shown. (D) Gephyrin or NSF immunoreactivity was normalized to actin immunoreactivity and it is presented as the mean ± S.E.M. of 4–7 independent experiments. TrkB-Fc prevented the BDNF-dependent increase in gephyrin protein expression (*p<0.05 or **p<0.01 compared to BDNF treated cultures by ANOVA).