Table 6.
Comparison of the Expression Values Between Microarray and qRT-PCR, Indicating the FC Between the Outer and Intermediate Interzones, as well as the Normalized Signal Intensity of the Microarray and the qRT-PCR
| 15.5 OI vs. 15.5 II | 15.5 II | 15.5 OI | 15.5 EC | |||||
|---|---|---|---|---|---|---|---|---|
| Gene | Microarray | PCR | Microarray | PCR | Microarray | PCR | Microarray | PCR |
| Col2a1 | 7.72 | 18.98 | 7959.63 | 6.27E-02 | 61459.29 | 1.19E+00 | 154205.97 | 1.53E+01 |
| Gdf5 | −1.03 | −10.74 | 4367.74 | 1.60E-03 | 4256.10 | 1.49E-04 | 1408.85 | 6.03E-06 |
| Matn1 | 41.47 | 69.61 | 6.74 | 7.70E-07 | 279.58 | 5.36E-05 | 23579.76 | 7.17E-02 |
| Arhgdib | −1.57 | −22.88 | 693.91 | 1.08E-02 | 442.80 | 4.72E-04 | ||
| Cytl1 | 65.26 | 310.98 | 135.55 | 3.28E-04 | 8845.57 | 1.02E-01 | ||
| Hhip | 9.84 | 3.36 | 23.7 | 3.15E-04 | 233.16 | 1.06E-03 | ||
| Lilrb3 | −28.2 | −2.35 | 118.62 | 5.82E-06 | 4.21 | 2.48E-06 | ||
For all genes, which had more than one probe included in the microarray, an average of the two or three replicate probes was calculated for inclusion in this table. Since the RNA was amplified differently for the two techniques, it is not surprising that the FCs are not identical between the two techniques. However the direction and degree of FCs are similar. For the genes used for verification of the layer selection, Gdf5, Col2a1, and Matn1, the normalized signal intensities measured in transient EC using microarray and qRT-PCR are also listed.
EC, embryonic cartilage; qRT-PCR, quantitative real-time polymerase chain reaction.