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. Author manuscript; available in PMC: 2014 Aug 4.
Published in final edited form as: Am J Transplant. 2014 Jun 16;14(7):1581–1591. doi: 10.1111/ajt.12763

Figure 5. Subclasses of serum IgG reactive to apoptotic cells.

Figure 5

(A) Example of subclass analysis by flow cytometry of IgG reactive to apoptotic Jurkat cells purified from 4 representative pre-transplant serum specimens, using secondary antibodies specific to IgG1 ~ IgG4. Filled gray histograms show background reactivity obtained with secondary antibodies alone. IgG1 ~ IgG4 reactivity to apoptotic Jurkat cells is depicted as solid line histograms. (B) Heat-map representation of IgG1 ~ IgG4 reactivity to apoptotic cells for the 50 most reactive serum samples. The shade of gray corresponds to the level of reactivity (MFI) as reported on the scale.