Skip to main content
. Author manuscript; available in PMC: 2014 Aug 4.
Published in final edited form as: Cell Rep. 2012 Mar 19;1(4):309–316. doi: 10.1016/j.celrep.2012.02.006

Figure 4. Expression of UBA3 A171T is sufficient to confer MLN4924 resistance.

Figure 4

(A) HCT116 cells transfected with the indicated plasmids were either treated with DMSO (-MLN4924) or 3 µM MLN4924 for 72 hours prior to crystal violet staining. (B) The expression of FLAG-tagged UBA3 was examined by immunoblot of UBA3 and FLAG in transfected HCT116 cells. GAPDH was used as a loading control. (C) Expression of UBA3 A171T increases HCT116 cells survival in the presence of increasing concentrations of MLN4924. Cell viability measurements for HMS and HMR cells treated with MLN4924 are shown for comparison.