Phylogenetic relationships of multi-gene families expressed in parasitism-specific tissues. Relatedness of (A) Ci-48a-like proteins and (B) reprolysin-like metalloprotease domain-containing proteins and their expression in M. demolitor tissues. Groups of teratocyte-specialized loci are indicated in grey shaded boxes, and venom-specialized groups are in white. Venom or teratocyte data sets are indicated in bold type, asterisks indicate detection in both. The plus sign indicates a gene product detected in both venom and teratocytes but does not belong to the venom set due to not meeting expression requirements. Support values for 100 bootstrap replicates are shown at nodes in the tree for clades supported by >80 replicates. Expression values (RPKM) for each locus in ovaries, larvae, teratocytes or venom glands are shown using a heat-map. Other species in the phylogeny are hymenopteran species Am: Apis mellifera, Ci: Chelonus inanitus, Ep: Eulophus pennicornis, Md: Microctonus hyperodae, Nv: Nasonia vitripennis, and Ph: Pimpla hypochondriaca, and dipteran species Dm: Drosophila melanogaster, Gm: Glossina moristans, and Sn: Simulium nigrimanum. Not enough sequence was available for H. didymator Hd-Ven1 and Hd-Ven2 metalloproteases or Microctonus aethipoides Ci-48a to be included. Microctonus and Venturia canescens peptidases also belong to a different family of proteases to those featured here (Crawford et al. 2003; Asgari et al. 2002).