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. 2014 Jul 1;15:25. doi: 10.1186/1471-2121-15-25

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Copyright © 2014 Müller-Taubenberger et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cells lacking NdrC show centrosomal aberrations. A. In comparison to wild-type, B, C multinucleated ndrC-null cells frequently show supernumerous centrosomes. D. Histogram depicting the percentage of normal (centrosomes/nuclei = 1) and aberrant (centrosomes/nuclei > 1) centrosomes in wild-type and ndrC-null cells. E, F. Visualization of mitotic spindles in fixed ndrC-null cells by immunolabeling with anti-α-tubulin antibodies, and staining of DNA with TO-PRO-3. Spindle formation occurs synchronously in multinucleate cells (E), as well as in multinucleate cells with supernumerous centrosomes (F). Bars 5 μm in (A), and, 10 μm in (B), (C), (E), and (F).

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