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. Author manuscript; available in PMC: 2015 Jun 5.
Published in final edited form as: J Immunol Methods. 2014 Jun 5;408:123–131. doi: 10.1016/j.jim.2014.05.014

Fig. 3.

Fig. 3

The impact of Nucleofection® on the expression of CD4 T cell surface activation markers. 5x106 primary human CD4 T cells were transfected with 2.5 μg of the empty control vector, Rc/CMV, and rested for 2 hours. Transfected cells (Amaxa, solid line) were then cultured with or without 2.5 μg/ml of PHA for 24 hours before being stained for CD25, CD69, CD154, and HLA-DR expression. As controls, non-transfected CD4 T cells (dashed line) were cultured for 24 hours with or without PHA before being stained for surface markers. Results are representative of three similar experiments.