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. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Cell. 2014 Jul 3;158(1):198–212. doi: 10.1016/j.cell.2014.04.045

Figure 3. Enhanced acquisition of repetitive motor routines requires NL3 expression in striatal D1-MSNs but not D2-MSNs.

Figure 3

(A) Illustration of genetic cross used to specifically delete NL3 from D1-MSNs.

(B-E) Rotarod performance of NL3-cKO mice without (n=16) and with D1-Cre (n=12). Time to fall off is presented at 4 to 40 rpm (B) and 8 to 80 rpm (C); the terminal speed of rotation (D) was used to calculate initial coordination and learning rate (E).

(F & G) Behavior of the same mice in a test of open field activity, showing time course of activity across the entire session (F) as well as total distance travelled and number of ambulatory episodes (G).

(H-N) Behavior of NL3-cKO mice without (n=8) and with A2a-Cre (n=10), which directs Cre expression exclusively to D2-MSNs.

(O) Illustration of cytosol aspiration from individual D1- and D2-MSNs of the NAc (top), and quantitative RT-PCR results from individual cells showing mRNA expression of cell type-specific markers (bottom).

(P-R) Relative mRNA expression quantified as the ratio between D1- and D2-MSNs, showing cell type-specific markers in the NAc (P) as well as neuroligins in the NAc (Q) and dorsal striatum (R).

Data are means ± SEM; *significant difference between groups (ANOVA). Also see Figures S3 & S4.