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. 2014 Jul 14;193(4):1544–1548. doi: 10.4049/jimmunol.1302108

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Copyright © 2014 by The American Association of Immunologists, Inc.

This article is distributed under The American Association of Immunologists, Inc., Reuse Terms and Conditions for Author Choice articles.

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FIGURE 1. — Identification of linear Ab epitopes in HERV-K (HML-2) TM expressed by infected cells. (A and B) Immunofluorescence. Representative images of HERV-K TM surface expression on infected TZMbl cells. Original magnification ×40. The detection of TM protein surface expression was performed by immunofluorescence on in vitro HIV-1_LAI–infected TZMbl cells 2 d postinfection. (A) Red: mouse anti–HERV-K (HML-2) TM; green: HA-137; blue: nucleus. (B) Green: HA-137; blue: nucleus (left panel). Blue: X-gal staining (right panel). Arrows represent coexpression of HIV-1 Tat protein (right panel) and HA-137 epitope (left panel). (C) Representative flow cytometry plots depicting HA-137 extracellular binding on infected PBMCs. HA-137 recognition was assessed by measuring the frequency of HA-137Alexa Fluor 488⁺ cells gated on live HIVGag⁺ or HIVGag⁻ cells from infected PBMCs. (D) Cumulative data from three independent experiments. The horizontal line represents the median in each group. The p value was derived by the Mann–Whitney T test.