Figure 6.

Effects of LTL on heat-killed E. coli induced inflammatory cell accumulation in BALF. BALF was prepared from mice 12 h after heat-killed E. coli O18:K1, 10⁴ CFU challenge with the induction of LTL at doses LTLD1 (25 mg/kg) and LTLD2 (50 mg/kg). Total cell counts (a), number of macrophages (b), neutrophils (c), and total protein (d) in BALF samples. LTLD1 and LTLD2 reduced the elevated level of inflammatory cytokines in BALF of E. coli challenged mice. BALF was collected at 12 h following E. coli challenge to assess the inflammatory cytokines such as TNF-α (e), IL-1β (f), IL-6 (g), and IL-10 (h) by ELISA and the chemokines CXCL5 and CXCL8 (i) by flow cytometry. Data are shown as means ± S.E.M. (*P < 0.05, **P < 0.01).