FIG. 10.

Hyperbaric oxygen preconditioning (HBO-PC) induces the up-regulation of anti-nuclear factor erythroid 2-related factor 2 (Nrf2) downstream gene expression in astrocytes of co-culture. (A) The mRNA levels of glutamate-cysteine ligase (GCL), γ-glutamyltransferase (γGT), and multidrug resistance protein 1 (MRP1) in astrocytes measured by real-time polymerase chain reaction were all significantly enhanced at 24 h after HBO-PC (**p<0.01 vs. CON group), but there is no change in neurons. (B) Using the housekeeping gene β-actin as an internal standard, the protein levels of GCL, γGT, and MRP1 measured by Western blot were significantly increased in astrocytes at 24 h after HBO-PC (**p<0.01 vs. CON group), but there is no change in neurons.