FIG. 4.
Hyperbaric oxygen preconditioning (HBO-PC) induces nuclear factor erythroid 2-related factor 2 (Nrf2) activation in mouse spinal cord (mean±standard deviation, n=6, **p<0.01 vs. CON group). (A) The Nrf2 mRNA level of spinal cord measured by real-time polymerase chain reaction was significantly enhanced at 12 h and 24 h after a single HBO-PC. (B) Using the housekeeping gene β-actin as an internal standard, the Nrf2 protein expression of spinal cord was also significantly increased measured by Western blot at 12 h and 24 h after a single HBO-PC. (C) Add 10 μg nuclear extract/well from the mice of each group, the relative DNA-binding activity of Nrf2 was determined using an enzyme-linked immunosorbent assay-based kit. The DNA-binding activity of nuclear Nrf2 significantly increased at 12 h and 24 h after a single HBO-PC.