FIG. 5.

Hyperbaric oxygen preconditioning (HBO-PC) induces the up-regulation of nuclear factor erythroid 2-related factor 2 (Nrf2) downstream gene expression. (A) The mRNA expression levels of glutamate-cysteine ligase (GCL), γ-glutamyltransferase (γGT), and multidrug resistance protein 1 (MRP1) measured by real-time polymerase chain reaction were significantly enhanced at 12 h and 24 h after a single HBO-PC (**p<0.01 vs. the CON group). (B) Using the housekeeping gene β-actin as an internal standard, the protein expression levels of GCL, γGT, and MRP1 in spinal cord tissues measured by Western blot were also significantly increased at 12 h and 24 h after a single HBO-PC (**p<0.01 vs. the CON group).