FIG. 7.

Representative images of spinal cord sections stained with anti-nuclear factor erythroid 2-related factor 2 (Nrf2) and the astrocyte marker glial fibrillary acidic protein (GFAP) or the neuronal marker anti-neuronal nuclei (NeuN) by double-immunofluorescence labeling. Scale bar=20 μm. At 24 h after hyperbaric oxygen preconditioning (HBO-PC), the fluorescence intensity of Nrf2 immunoreactivity was significantly increased in GFAP positive astrocytes, and there is only a slight enhancement of Nrf2 immunoreactivity in NeuN positive neurons.