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. 2014 Aug 1;3(4):162–175. doi: 10.1089/biores.2014.0019

FIG. 1.

FIG. 1.

Timeline of neural differentiation and the auditory neuron developmental markers examined in vitro. (A) The hiPSCs and hESCs (control) were treated with Noggin media supplemented with bFGF for 2 weeks (neural induction). Subsequently, dissected colony pieces were treated with EGF and bFGF (neurosphere induction). The neurospheres were plated on a layer of HFF and treated with EGF and bFGF at 18 DIV, followed by treatment with Y27632 at 19 and 21 DIV (neural crest induction). From 24 DIV onward, the neurospheres were cultured in NBM only (sensory neural induction). Note: The hiPSCs and hESCs were analyzed for AN marker expression using immunochemical and qRT-PCR techniques at the time points highlighted in red. (B) The transcription factors analyzed at different stages of AN development. AN, auditory neuron; bFGF, basic fibroblast growth factor; DIV, days in vitro; EGF, epidermal growth factor; hESCs, human embryonic stem cells; HFF, human foreskin fibroblasts; hiPSCs, human induced pluripotent stem cells; NBM, neurobasal media; qRT-PCR, quantitative real time polymerase chain reaction.