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. 2014 Aug 1;3(4):162–175. doi: 10.1089/biores.2014.0019

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 5. — Expression of ßIII-tubulin and NFM in the hiPSC- and hESC-derived neurospheres. (A) The neural specific markers ßIII-tubulin and NFM are expressed in the ganglion neuron and at AN stages of development. (B) Both the hiPSC and hESC neurospheres showed some level of ßIII-tubulin expression over the time course examined. Note that at 35 DIV, the levels of ßIII-tubulin had declined in the hiPSC line iPS2 and hESC line H9 cell lines (arrows). Scale bars=100μm (relative to all images). (C) Bipolar BRN3A+/NFM+ neurons were observed from as early as 24 DIV in the hiPSC and hESC-derived neural progenitors (arrows). Scale bar=50 μm (relative to all images). NFM, Neurofilament kDa 160.