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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Exp Cell Res. 2014 May 28;326(1):78–89. doi: 10.1016/j.yexcr.2014.05.018

Figure 5.

Figure 5

Reduced cell migration and growth of MDA-MB-231 cells by repressing FAK or HSP90β expression. (A, B) Inhibition of FAK and HSP90β expression by miRNAi constructs. MDA-MB-231 cells were transfected with miRNAi constructs of FAK or HSP90β. The control miRNAi encodes scrambled sequences. Forty-eight hours after transfection, cell lysates were immunoblotted with antibodies against FAK (A), HSP90β (B), or GAPDH for loading control. (C, D) Migration of cells transfected with miR-FAK1763 or miR-90β-1205 were reduced. Cell migration was quantified by measuring the difference in distance between the leading edge at the initiation of the experiment and after 12, 18, 24 h of incubation. (E, F) Anchorage-independent growth of MDA-MB-231 cells expressed miR-FAK1763 or miR-90β-1205 were evaluated by the CFE as histograms. Colonies with more than 50 cells were counted by light microscopy. CFE is determined by formula as: (number of colonies formed/number of cells incubated) × 100%. Scale bar indicates 200 μm. Error bars represent the standard deviation of three independent experiments. ** P < 0.01, compared with control groups.