Figure 5.

GNeo-QD525 conjugate colocalizes with lysosomes. Wild-type Chinese hamster ovary cells were incubated with 5 nmol/L GNeo-QD525 in growth medium for 30 min. After the cells were rinsed three times, fresh medium was added, and 2.5 h later, they were rinsed with Hank’s balanced salt solution and labeled with Hoechst dye and LysoTracker Red. Images were captured with a DeltaVision Restoration microscope system and were deconvolved to show the localization of (a) GNeo-QD525 and (b) lysosomes in a single Z-stack plane. The merged images from (a) and (b) are shown in (c) with the outline of cells (hatched line) drawn based on a phase contrast micrograph. Reprinted with permission from ref (46). Copyright 2010 Nature Publishing Group.