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Confocal imaging and 3D reconstruction of an enteroid at low (A) and high magnification (B: outlined area in A). Images show transmitted light, nuclei (blue), E-cadherin (green), paneth cell (pink), and ZO1-RFP (red). RFP (ZO-1) is endogenously expressed while E-cadherin is detected using a specific antibody. Paneth cells are marked by nonspecific staining of Alexa Fluor 488 F(ab′)2 fragment of goat anti-rabbit IgG. Nuclei are labeled with Hoechst 33342.
