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Chromatogram of Jiubiying extracts by semi-preparative HSCCC. Conditions: solvent system: ethyl acetate-n-butanol-water (1:6:7, v/v/v); stationary phase: upper organic phase (head-tail mode); mobile phase: lower aqueous phase (head-tail model); column volume: 260 mL; sample concentration: 25 mg/mL; sample volume: 12 mL; flow rate: 1.0 mL/min; revolution: 850 rpm; detection wavelength: 254 nm; temperature: 25oC; retention of stationary phase: 35%.
