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Chromatogram of Jiubiying extracts by preparative HSCCC. Conditions: solvent system: ethyl acetate-n-butanol-water (1:6:7, v/v/v); stationary phase: upper organic phase (head-tail mode); mobile phase: lower aqueous phase (head-tail mode); column volume: 1000 mL; sample concentration: 25 mg/mL; sample volume: 40 mL; flow-rate: 3.0 mL/min; revolution: 450 rpm; detection wavelength: 254 nm; temperature: 25oC; retention of stationary phase: 50%.
