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. Author manuscript; available in PMC: 2014 Aug 4.
Published in final edited form as: Nat Methods. 2009 Nov;6(11 0):S22–S32. doi: 10.1038/nmeth.1371

Figure 5. Overview of RNA-seq.

Figure 5

A RNA fraction of interest is selected, fragmented, and reverse transcribed. The resulting cDNA can then be sequenced using any of the current ultra-high-throughput technologies to obtain ten to a hundred million reads, which are then mapped back onto the genome. The reads are then analyzed to calculate expression levels.