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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2014 Feb 27;34(5):1020–1031. doi: 10.1161/ATVBAHA.114.303403

Figure 3. Effect of MKP-1 depletion on in vitro angiogenesis.

Figure 3

HUVEC were depleted of MKP-1 by siRNA (A) and serum starved 2hrs prior to treatment with 50 ng/ml VEGF. VEGF-induced migration (B, C), proliferation (D) and tube formation (E, F) were evaluated using the scratch wound, BrdU, and Matrigel assays, respectively. Representative migration images in (B) show initial wound (top) and migration over 11 hrs (bottom) in VEGF-treated samples. Representative tube formation images in (E) show tube formation over 6 hrs after VEGF treatment. MKP-1 depletion abrogated VEGF-induced migration and tube formation and reduced overall proliferation but did not block the pro-proliferative effect of VEGF. *p ≤ 0.05, **p ≤ 0.01, NS = not significant. Scale bars: 100 μm.