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. Author manuscript; available in PMC: 2014 Aug 4.
Published in final edited form as: Anat Rec (Hoboken). 2010 Jun;293(6):968–981. doi: 10.1002/ar.21094

Figure 6.

Figure 6

Syndecan-1/KC Complexes control the pulmonary recruitment of neutrophils in mice treated with bleomycin. (A) KC was immunoprecipitated (IP) from BAL of WT mice 1 day post-bleomycin. BAL and post-IP supernatants and pellets were electrophoresed and blotted for syndecan-1 ectodomain. The arrowhead indicates the band specifically coprecipitated with KC. (B) KC and syndecan-1 were immunoprecipitated from BAL of WT mice 1 day post-bleomycin. Two additional syndecan-1 IPs were done on the post-IP supernatant. The level of KC protein in the BAL and in the post-IP supernatants was quantified by ELISA. (C–E) WT and syndecan-1 null mice (SYN1−/−) (n = 3) were instilled with 0.15 U bleomycin. KC levels in BAL (C) and lung homogenates (D) were determined by ELISA. (E) Neutrophils (PMNs) in BAL were counted and expressed as a percent of total leukocytes. Data are the mean ± SE.. (This figure is from studies by Li, et al., (Li et al., 2002) with permission.)