Figure 1. Protein interaction and gene expression analyses of SNF1 kinase complex components and its activating kinases in M. oryzae.
(A) Different composition of the heterotrimeric SNF1 kinase complex and upstream kinases between S. cerevisiae and M. oryzae. (B) MoSnf1, MoSip2, and MoSnf4 interacted with each other, while no interaction was observed between MoSnf1 and its activating kinases in yeast two-hybrid assay. Yeast transformants expressing MoSnf1 plus MoSip2, MoSnf1 plus MoSnf4, MoSip2 plus MoSnf4, MoSnf1 plus MoSak1, or MoSnf1 plus MoTos3 were 10-fold serially diluted with a starter culture of 106 cells/ml and then spotted (5 µl) onto SD-Trp-Leu-His-Ade medium. (C) Gene expression profiles of MoSNF1, MoSIP2, MoSNF4, MoSAK1, and MoTOS3 among different developmental stages. Tested fungal tissues included vegetative hyphae (VH), conidia (CO), appressoria 8 hpi (AP), and invasive hyphae (72 hpi), which were within infected plant leaves (IP). Gene expression data, obtained from quantitative RT-PCR analysis, were normalized by using β-tubulin as an internal control and calibrated against the transcript abundances of VH stage.