Figure 7. Indirect assessment of turgor pressure and appressorial porosity.

(A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying concentrations (1–4 M). (B) Plasmolysis/cytorrhysis assay with osmotic solutions of different average molecular weights. The solutions were adjusted to the denoted concentrations to exert 4 MPa osmotic pressure on appressoria at 48 hpi. (C) Plasmolyzed appressoria at 48 hpi were photographed after soaked in 1.7 M glycerol solution for 10 min. Bars  = 5 µm.