Figure 8. Pathogenicity assay on detached barley leaves.
Intact and abraded barley leaves were inoculated with 20 µl conidial suspensions (1×105 conidia/ml) of the tested strains for 4 days before photography. ΔMosnf1, ΔMosip2, ΔMosnf4, ΔMosak1, and ΔMosak1ΔMotos3 were deficient in appressorium-mediated infection of intact barley leaves. Elevated virulence was observed in ΔMosip2, ΔMosnf4, and ΔMosak1 when tested on abraded barley leaves, while ΔMosnf1 and ΔMosak1ΔMoto3 were still non-pathogenic. Addition of 2.5% glucose to conidial suspensions could evidently, yet partially, restored the virulence of the defective mutants.