Table 3.
Genes involved in the metabolism of cholesterol by M. tuberculosis and the experiments conducted for each gene.
| Gene name | Mtb gene number | Putative function | Studies performed | Reference |
|---|---|---|---|---|
| hsd | Rv1106c | 3β-hydroxysteroid dehydrogenase | B, C, D, E, G, H | 36, 37 |
| kstD | Rv3537 | ketosteroid Δ1-dehydrogenase | B, C, D, E, H | 38, 93 |
| kshA | Rv3526 | 3-Ketosteroid 9α-hydroxylase | B, D, E, F, H | 39, 94, 95 |
| kshB | Rv3571 | 3-Ketosteroid 9α-hydroxylase | B, D, E, F, H | 39, 94, 95 |
| hsaA | Rv3570c | 3-HSA-hydroxylase | B, D, E, H | 40 |
| hsaB | Rv3567c | 3-HSA-hydroxylase | B, D, E | 40 |
| hsaC | Rv3568c | 2,3-dehydroxyphenyl dioxygenase | B, E, F, H | 41 |
| hsaD | Rv3569c | 4,9-DSHA hydrolase | B, E, H | 96 |
| hsaE | Rv3536c | hydratase | A, E | 42 |
| hsaF | Rv3534c | aldolase | A, E | 42 |
| hsaG | Rv3535c | acetaldehyde dehydrogenase | A, E | 5 |
| cyp125 | Rv3545c | cytochrome P450 | B, D, F | 50, 51, 54, 97-99 |
| cyp142 | Rv3518c | cytochrome P450 | B | 52, 100 |
| fadD3 | Rv3561 | acyl-CoA synthetase | D, E | |
| fadD17 | Rv3506 | acyl-CoA synthetase | A,E | |
| fadD18 | Rv3513c | acyl-CoA synthetase | A,E | |
| fadD19 | Rv3515c | acyl-CoA synthetase | D, E | |
| fadD36 | Rv1193 | acyl-CoA synthetase | D | |
| fadE5 | Rv0244c | acyl-CoA dehydrogenase | D, E | |
| fadE14 | Rv1346 | acyl-CoA dehydrogenase | E | |
| fadE17 | Rv1934c | acyl-CoA dehydrogenase | E | 54, 55, 99 |
| fadE18 | Rv1933c | acyl-CoA dehydrogenase | E | 54, 55, 99 |
| fadE25 | Rv3274c | acyl-CoA dehydrogenase | D | |
| fadE26 | Rv3504 | acyl-CoA dehydrogenase | E | |
| fadE27 | Rv3505 | acyl-CoA dehydrogenase | E | |
| fadE28 | Rv3544c | acyl-CoA dehydrogenase | B, C, D, E, F | 54, 55, 99 |
| fadE29 | Rv3543c | acyl-CoA dehydrogenase | B, C, D, E, F | 54, 55, 99 |
| fadE30 | Rv3560c | acyl-CoA dehydrogenase | D, E | |
| fadE31 | Rv3562 | acyl-CoA dehydrogenase | D, E | |
| fadE32 | Rv3563 | acyl-CoA dehydrogenase | D, E | |
| fadE33 | Rv3564 | acyl-CoA dehydrogenase | D, E | |
| fadE34 | Rv3573c | acyl-CoA dehydrogenase | D, E | |
| echA9 | Rv1071c | (S)-enoyl-CoA hydratase | D | |
| echA19 | Rv3516 | (S)-enoyl-CoA hydratase | E, G | |
| echA20 | Rv3550 | (S)-enoyl-CoA hydratase | E | |
| Rv3541c | (R)-enoyl-CoA hydratase | C, D, E, F | 54, 55, 99 | |
| Rv3542c | (R)-enoyl-CoA hydratase | C, D, E, F | 54, 55, 99 | |
| fadA5 | Rv3546 | thiolase | B, C, D, E, F | 34 |
| fadA6 | Rv3556c | thiolase | E |
. Bioinformatic annotation
. Recombinant expression and enzymatic function confirmed
. Function examined with mutant strain in vivo
. Required for growth on cholesterol 35
. Upregulated by cholesterol 34
. Growth phenotype in vivo
. No growth phenotype in vivo
. Growth phenotype in macrophages 101