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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Reproduction. 2014 Mar 24;148(1):1–10. doi: 10.1530/REP-13-0548

Figure 3.

Figure 3

Representative image of immunolocalization of PrPC protein in endometrium of E2-treated OVX ewes. Arrows indicate PrPC-positive (dark) staining; counterstaining (pink) was with nuclear fast red. Note a lack of positive staining in control (inset), where primary antibody was replaced with mouse IgG. L = lumen of uterus, g = uterine gland, * = blood vessel. Note that PrPC was expressed in arterioles and venules (primarily vascular smooth muscle and adventitia) at 0 to 24 h after E2, but PrPC was not present in glands or luminal epithelium. Size bar = 50 μm.