Figure 5.
Expression of PrPC protein in caruncle (CAR; A) and fetal membranes (FM; B) during early pregnancy in ewes. A representative membrane of the Western blots is shown on the top of the graph, where the tissues and samples are shown in the same order as they are on the bar graph below. PrPC protein had a molecular weight between 30 and 45 kd. Note that PrPC protein was a single band in CAR, but was expressed as two protein bands in FM. NS = not significant; a,bP means ± S.E.M with different superscripts differ as indicated on the graph. Values are from densitometry of the Western blots. The pattern of change in FM for PrPC mRNA (Fig. 4) and protein expression closely paralleled each other.