(A) Southern blot hybridization of genomic DNAs of S. paucimobilis B90A, B90, Sp+, and UT26 digested with BamHI and hybridized with [α-32P]dATP-labeled IS6100. Lane 1, Gene Ruler DNA ladder mixture; lane 2, B90A; lane 3, B90; lane 4, Sp+; lane 5, UT26. (B) Southern blot hybridization of PstI-digested genomic DNAs of S. paucimobilis B90A, B90, Sp+, and UT26 hybridized with [α-32P]dATP-labeled linD (panel a) and linE (panel b) as probes. Lane 1, Gene Ruler DNA ladder mixture; lane 2, B90A; lane 3, B90; lane 4, Sp+; lane 5, UT26. (C) Southern blot hybridization of genomic DNAs of S. paucimobilis Sp+ mutants digested with HindIII and BamHI and hybridized with [α-32P]dATP-labeled IS6100 (panel a) and linA (panel b). Lane1, Gene Ruler DNA ladder mixture; lanes 2 to 6, HindIII-digested genomic DNAs of Sp+ and mutants 1 to 4; lanes 7 to 11, BamHI-digested genomic DNAs of Sp+ and mutants 1 to 4.