TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Description | Source |
|---|---|---|
| E. coli DH5α | F−endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR | Lab stock |
| E. coli JM101 | supE thiΔ(lac-proAB) F′[traD36 proAB+lacIqlacZΔM15] | Lab stock |
| S. paucimobilis B90A | Aerobic, nonmotile, rod shaped, degrades all four isomers of HCH (α, β, γ, and δ) | N. Sethunathan, CRRI, Cuttack, India |
| S. paucimobilis B90 | Mutant of S. paucimobilis B90A lacking linD, linE, and linR | Lab stock |
| S. paucimobilis UT26 | Aerobic, nonmotile, rod shaped, degrades α-, γ-, and δ-HCH but not β-HCH | Y. Nagata, University of Tokyo, Tokyo, Japan |
| S. paucimobilis Sp+ | Aerobic, nonmotile, rod shaped, degrades α-, γ-, and δ-HCH but not β-HCH | Tim Vogel, University of Lyon, Lyon, France |
| S. paucimobilis ATCC 29837T | Aerobic, nonmotile, rod shaped, yellow pigmented | M. Hanspal, St. Elizabeth's Medical Center of Boston, Boston, Mass. |
| S. chlorophenolica DSM 7098T | Aerobic, nonsporulating rods, yellow pigmented, degrades pentachlorophenol, and 2,4,6-trichlorophenol | J. Cullum, University of Kaiserslautern, Kaiserslautern, Germany |
| pUC 13/19 | 2.7-kb; Ampr; multiple cloning site internal to lacZ gene | Lab stock |
| pWE15 | 8.2-kb cosmid vector; Ampr; bacteriophage promoter sequences T3 and T7 flanking unique cloning site | Stratagene |
| pLINA57 | pWE15 carrying 41-kb DNA fragment of B90A containing linA1, linC, linX, and IS6100 | This study |
| pLIND33 | pWE15 carrying 5.6-kb DNA fragment of B90A containing linD, linE, linR, and IS6100 | This study |
| pLIND22 | Subclone of pLIND33 in pWE15 (bp 1869 to 6700 of the contiguous ∼8-kb sequence of pLIND33) | This study |
| pLIND22-12 | Subclone of pLIND22 in pUC18 (bp 6701 to 7803 of the contiguous ∼8-kb sequence of pLIND33) | This study |
| pLIND3R24 | Subclone of pLIND33 in pUC18 (bp 1 to 1868 of the contiguous ∼8-kb sequence of pLIND33) | This study |
| pLINB35 | pWE15 carrying DNA fragment of B90A containing linB and IS6100 | This study |
| pLINB23 | Subclone of pLINB35 in pUC18 containing 2.2-kb fragment of pLINB35 | This study |
| pLINUTIS | pUC 18 containing BamHI-digested 3.5-kb fragment of S. paucimobilis UT26 that hybridized with IS6100 | This study |
| pLINSpIS | pUC18 containing HindIII-digested 2.2-kb Sp+ fragment that hybridized with IS6100 | This study |