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. 2014 Aug 4;9(8):e102902. doi: 10.1371/journal.pone.0102902

Figure 2. RBFD253A is pro-apoptotic in the ZNC and induces more apoptosis than RBF in third instar larvae wing imaginal discs.

Figure 2

(A, E) C96-Gal4 and vg-Gal4 expression patterns are visualized by UAS-mtGFP expression in third instar larvae wing imaginal discs. (B-D, F-H) Apoptotic cells are labeled by TUNEL in wing imaginal discs; specific staining of apoptotic cells corresponds to bright white patches. (B, F) C96-Gal4/+ and vg-Gal4/+ control discs have few apoptotic cells. (C) C96-Gal4/UAS-RBF wing discs are similar to control. (D) Some apoptotic cells are observed within the C96-Gal4 expression domain in UAS-RBFD253A/X; C96-Gal4/+ discs (white arrow). (G, H) Apoptotic cells are observed within the vg-Gal4 expression domain in vg-Gal4/+; UAS-RBF/+ and UAS-RBFD253A/X; vg-Gal4/+ wing discs (white arrows). (G) The white arrowhead indicates a zone at the center of the pouch where cells are not TUNEL-labeled in vg-Gal4/+; UAS-RBF/+ wing discs. All discs are shown with posterior to the top.