Figure 3. The change in free energy and likelihood across the reactions shown in Scheme 1.

A. The equilibrium position is where the total free energy change (solid line) crosses the ordinate at 0.0 Kcal Kcal/mol and the abscissa at 0.26 n_B/N_total. In accordance with detailed balance, the free energy change for each reaction is also 0 Kcal Kcal/mol. B. The combined likelihood for the reactions (solid line) is 1.0 at equilibrium, just as the likelihoods for each reaction individually.