TABLE 1.
Efficiencies of plating of red gam λ bacteriophage on RecA+ (JM105) and RecA− (JM109) E. coli cells containing expression plasmids bearing E. coli, L. biflexa, B. burgdorferi, and B. hermsii recA genes
| Strain/plasmid | recA gene | No. of PFU/platea | Mean no. of plaques (95% CI)b | % Compared to JM105 |
|---|---|---|---|---|
| JM105/none | E. coli | 500 | 514 (454-572) | 100 |
| JM109/pBluescript | None | 15,000 | 0 | <0.006 |
| JM109/pEc:recA | E. coli | 1,500 | 979 (866-1092) | 65 |
| JM109/pLb:recA | L. biflexa | 1,500 | 374 (331-415) | 25 |
| JM109/pBb:recA | B. burgdorferi | 15,000 | 0 | <0.006 |
| JM109/pBh:recA | B. hermsii | 15,000 | 0 | <0.006 |
a
Estimated number of PFU per plate.
b
95% CI, 95% confidence interval.