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. Author manuscript; available in PMC: 2014 Aug 4.
Published in final edited form as: Nat Protoc. 2013 Aug 8;8(9):1670–1679. doi: 10.1038/nprot.2013.106

Figure 6.

Figure 6

Pasteur pipette technique for triturating MGE-like neurospheres. Flame the end of a cotton-filtered Pasteur pipette to round the edges and narrow the opening to about 0.2–0.5 mm in diameter. Flame the narrow part of the shaft to introduce a 15–20° smooth curve. Rinse the pipette wall with the culture medium three times. Pipette neuroepithelial clusters using the treated Pasteur pipette by pulling the cells in and push them out one or two times. The narrow opening and bend in the pipette will help shear the clusters into smaller pieces of a roughly uniform size. Do not triturate more than three times.