Figure 8.

Effect of ChoD on the phosphorylation of p38 MAPK. THP-1-derived macrophages were treated with ChoD (0.01–0.5 U/mL) for 24 h or left untreated (controls). Phosphorylation of kinases was assessed by flow cytometry. Data are expressed as means ± SEM of MFI values from 7-8 independent experiments (*P < 0.05, **P < 0.02 for macrophages + ChoD versus macrophages). Representative histograms demonstrate fluorescence intensity of total (b) and phosphorylated p38 MAPK (c). The differences in p38 MAPK phosphorylation in control macrophages as well as macrophages treated with ChoD (0.5 U/mL) are shown.